Effects of Storage Time and Exogenous Protease Inhibitors on Plasma Protein Levels Ayache et. al. american Journal of Clinical Pathology, 2006 vol. 126 (2) pp. 174-184.
This is an elegant study demonstrating the significant changes in a wide variety (~37) of biomarkers with a 2-hour hold prior to processing. The study also evaluates the influence of protease inhibitors sample behavior. Why we like this article: Beyond the issues of handling and short-term storage of plasma, the study provoked an excited discussion amongst BioCoR facutly who could see the implications of the study extending far beyond the conclusions made in the paper.
Listing of protective agents
Published on: Thursday 18, August 2011Category: Article of Interest
A listing of chemicals that have been associated with preservation:
acetamide, agaroses, alginates, alanine albumin, ammonium acetate, butanediol, chondroitin sulfate, chloroform, choline, cyclohexanediols**, dextrnas*, diethylene glycol, dimethyl acetamide, dimethyl formamide, dimethyl sulfoxide*, erythritol, lethano
Freezing of Tissue Specimens
Published on: Thursday 18, August 2011Category: Article of Interest
Freezing of Tissue Specimens: bibliography for Tissue Freezing Techniques
Preservation of Biofluids
Published on: Thursday 18, August 2011Category: Article of Interest
Hubel A, Aksan A, Skubiz APN, Wendt C, Zhong X, "State of the art in preservaion of fluid biospecimens", Biopreservation&Biobanking, 9(3): 237-244, 2011.
Why it is important: The state of the art in preservation of plasma/serum, urine, saliva, cerebrospinal fluid are reviewed with emphasis on the variables that in
Preservation of Nucleic Acids
Published on: Thursday 18, August 2011Category: Article of Interest
Anchordoquy TJ and Molina MC, "Preservation of DNA", Cell Preservation Technology, 5(4): 180-188, 2007.
Why it is important: It reminds us that preservation of DNA is not a completely solved problem. Freezing can damage DNA strands and make the sample less useful for future genomic techniques. A good revie
Economic Impact of Cell Therapy
Published on: Thursday 18, August 2011Category: Article of Interest
Mason C, Brindley DA, Culme-Seymour EJ, Davie NL, "Cell therapy Industry: billion dollar global buisness with unlimited potential", Regen Med 6(3); 265-272, 2011.
Why it is important: It demonstrates the manner by which tissue engineering/regenerative medicine has been eclipsed by cell therapy and the very real econom
Biospecimen identifiers
Published on: Monday 28, March 2011Category: Article of Interest
Nietfield JJ, Sugarman J, Litton JE, "The Bio-PIN: a concept to improve biobanking," Nature Reviews Cancer, 11: 303-308, 2011.
Why it is important: The paper proposes the structures of a method of identifying biospecimens based on single nucleotide polymorphisms. Issues of identification of samples and tracking
Biomarker Stability fo MMP-7, TIMP-1, VEGF and VEGF-R2
Published on: Monday 28, March 2011Category: Article of Interest
Kisland K, Kerna I, Kumm J, Jonsson, H, Tamm A, "Impact of cryopreservation on serum concentration of metalloptoteinases (MMP)-7, TIMP-1, vascular endothelial growth factor (VEGF) and VEGF-R2 in Biobank samples," Clin Chem Lab Med 49 (2): 229-235, 2011.
Why it is important: Studies on the stability of these biomarkers
Spatial Distribution of the State of Water in Frozen Mammalian Cells
Published on: Tuesday 02, November 2010Category: Article of Interest
Doug J, Malsam J, Bischof JC, Hubel A, Aksan A, "Spacial distribution of the state of water in frozen mammalian cells, Biophys J, 99: 2453-2459, 2010.
The direct determination of the state of intracellular water, mearsurement of the intercellular concentration of a cryoprotectant agent (dimethylsulfoxide), and the dis
Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions
Published on: Wednesday 23, June 2010Category: article of interest
Cao et al. Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions. Biotechnol. Bioeng. (2003) vol. 82 (6) pp. 684-90
The article clearly describes the importance of both cooling and thawing rates on the post thaw activity of proteins.
Storage conditions influence post thaw recovery
Published on: Thursday 11, February 2010Category: article of interest
Fowke, K.R., et al., Apoptosis: a method for evaluating the cryopreservation of whole blood and peripheral blood mononuclear cells. J Immunol Methods, 2000, 244(1-2): p. 139-44.
The article compares the influence of storage temperature on recovery of human whole blood samples.
