Biomarker Stability fo MMP-7, TIMP-1, VEGF and VEGF-R2
Published on: Monday 28, March 2011Kisland K, Kerna I, Kumm J, Jonsson, H, Tamm A, "Impact of cryopreservation on serum concentration of metalloptoteinases (MMP)-7, TIMP-1, vascular endothelial growth factor (VEGF) and VEGF-R2 in Biobank samples," Clin Chem Lab Med 49 (2): 229-235, 2011.
Why it is important: Studies on the stability of these biomarkers have addressed issues of collection process but not storage process on stability of the markers. The study also used accelerated aging to determine stability of markers with storage. Certain markers were very sensitive to repeated freeze-thaw cycles (VEGF-R2). Stability with storage was also determined and TIMP-1 and VEGF demonstrated very poor stability with storage at ~-80oC.
